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מבחני ביוכימיים לניתוח פעילויות של ATP תלוי הכרומטין שיפוץ אנזימים
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JoVE 신문 생물학
Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
DOI:

10:14 min

October 25, 2014

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Chapters

  • 00:05Title
  • 01:15ATP-dependent Nucleosome Remodeling Assays
  • 02:55ATP-dependent Nucleosome Sliding Assays
  • 05:11DNA- and Nucleosome-dependent ATPase Assays
  • 07:15Defining Biochemical Activities of the INO80 Complex and INO80 Subcomplexes
  • 09:17Conclusion

Summary

자동 번역

Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.

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