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In Vitro Pancreas Organogenese van Dispersed muis embryonale Progenitors
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JoVE 신문 생물학
In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors
DOI:

08:35 min

July 19, 2014

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Chapters

  • 00:05Title
  • 01:55Dissection of Dorsal Pancreatic Bud from Mouse Embryos
  • 04:19Plating and Culturing of Dispersed Cells
  • 06:06Results: In Vitro Expansion and Differentiation of Embryonic Pancreatic Progenitors
  • 07:20Conclusion

Summary

자동 번역

De driedimensionale cultuur methode beschreven in dit protocol recapituleert pancreas ontwikkeling van verspreide embryonale muis alvleesklier progenitoren, waaronder hun grote expansie, differentiatie en morfogenese in een vertakte orgaan. Deze methode is vatbaar voor beeldvorming, functiestoringen en manipulatie van de niche.

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