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Chapters
- 00:05Title
- 01:19Generation of Mouse Embryonic Fibroblasts (MEFs)
- 03:17Reprogramming of MEFs
- 05:14Antibody Labeling
- 06:50FACS Isolation of Intermediates
- 08:26Results: Representative Images and FACS Profiles of Reprogramming MEF Cultures
- 10:09Conclusion
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
