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High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
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1Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Microbial and Molecular Pathogenesis, College of Medicine,Texas A&M University System Health Science Center, 3University of California, Irvine, 4Department of Medical Microbiology & Immunology, School of Medicine,University of California, Davis
Chapters
- 00:05Title
- 01:59Murine Macrophage RAW264.7 Cell Culture
- 03:29Preparation of Salmonella Wild-type and Mutants
- 05:19Invasion Assay in a 96-well Culture Plate
- 09:58Results: Identification of the Phenotype of Salmonella Mutants
- 10:46Conclusion
A high-throughput assay to in vitro phenotype Salmonella or other bacterial association, invasion, and replication in phagocytic cells with high-throughput capacity was developed. The method was employed to evaluate Salmonella gene knockout mutant strains for their involvements in host-pathogen interactions.
