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Chapters
- 00:05Title
- 01:15Preparation of Larvae
- 02:08Coarse Dissection of Larvae
- 03:30Fixation and Staining of Tissue with Antibodies
- 06:17Fine Dissection of Eye-Antennal Disc Complexes and Mounting
- 08:11Results: Dynamic Images Produced for In-depth Analysis
- 09:39Conclusion
The adult structures of Drosophila are derived from sac-like structures called imaginal discs. Analysis of these discs provides insight into many developmental processes including tissue determination, compartment boundary establishment, cell proliferation, cell fate specification, and planar cell polarity. This protocol is used to prepare imaginal discs for light/fluorescent microscopy.
