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3D تتبع المداري في التعديل المجهر ثنائي الفوتون: دراسة تطبيقية على تتبع من بين الخلايا الحويصلات
JoVE 신문
생체공학
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JoVE 신문 생체공학
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
DOI:

11:28 min

October 01, 2014

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Chapters

  • 00:05Title
  • 01:50Sample Preparation
  • 03:19Microscope Configurations
  • 05:05Imaging
  • 07:21Trajectory Analysis
  • 08:40Results: Fast 3D Single Particle Tracking of an Isolated Lysosome Moving Inside the Cell After the Endosome Maturation Process
  • 10:36Conclusion

Summary

자동 번역

In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.

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