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Chapters
- 00:05Title
- 01:26Preparation of Modified C. elegans Habituation and Reproduction Medium (mCeHR)
- 02:50Preparing C. elegans for mCeHR Culture
- 05:39Synchronizing and Storing Worms in mCeHR Culture
- 06:55Results: Nutrient Studies and Trangene Delivery in mCeHR
- 07:51Conclusion
C. elegans is usually grown on solid agar plates or in liquid cultures seeded with E. coli. To prevent bacterial byproducts from confounding toxicological and nutritional studies, we utilized an axenic liquid medium, CeHR, to grow and synchronize a large number of worms for a range of downstream applications.
