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Assessing the Secretory Capacity of Pancreatic Acinar Cells
JoVE 신문
생물학
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JoVE 신문 생물학
Assessing the Secretory Capacity of Pancreatic Acinar Cells
DOI:

09:52 min

August 28, 2014

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Chapters

  • 00:05Title
  • 01:25Excision of the Murine Pancreas
  • 02:57Collagenase Digestion
  • 04:57Amylase Secretion Assay
  • 07:20Live Imaging of Pancreatic Secretion
  • 08:09Results: Assessing the Secretory Capacity of Pancreatic Acinar Cells
  • 09:10Conclusion

Summary

자동 번역

Isolated pancreatic acini retain their in vivo morphology and activity and offer powerful ways for monitoring and manipulating secretion. This work demonstrates how acini can be isolated from the mouse pancreas, and how their secretory capacities can be assessed.

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