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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
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Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

DOI:
10.3791/51814-v

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09:39 min

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August 21, 2014

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1Département de Génomique Fonctionnelle et Cancer,Institut de Génétique et de Biologie Moléculaire et Cellulaire, 2Center for Life NanoScience,Istituto Italiano di Tecnologia

Chapters

  • 00:05Title
  • 01:04Sample Collection
  • 01:46RNA Extraction Analysis
  • 03:04Acrylamide Gel and Sample Preparation
  • 03:58Electrophoretic Fractionation
  • 04:41Gel Blotting
  • 06:03Probe Synthesis, Membrane Crosslinking, and Prehybridization
  • 07:27Membrane Hybridization, Washing, and Signal Detection
  • 08:20Results: Improved RNA Detection Sensitivity
  • 09:06Conclusion

Summary

자동 번역

자동 번역

The aim of this publication is to visualize and discuss the operative steps of an Enhanced Northern Blot protocol on RNA extracted from Drosophila melanogaster embryos, cells, and tissues. This protocol is particularly useful for the efficient detection of small RNA species.

Tags

Keywords: Enhanced Northern BlotSmall RNAMicroRNADrosophila MelanogasterGene ExpressionPost-transcriptional RegulationNoncoding RNARNA ProfilingRNA DetectionMolecular BiologyEmbryoCell Culture

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