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Chapters
- 00:05Title
- 01:24Preparing Tissue Slices
- 04:10Time-Lapse Imaging of NG2 Cell Division
- 05:39Slice Immunohistochemistry
- 07:17NG2 Cell Differentiation and Mature Oligodendrocyte Characterization
- 09:08Conclusion
A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.
