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Chapters
- 00:05Title
- 01:16Gel Excision and Removal of Gel-derived Contaminants
- 03:14In-gel O-glycan Release by Reductive β-Elimination and Desalting on Cation-exchange Chromatography
- 06:02Borate Removal, C18 Clean-up and Base Preparation for Permethylation
- 08:03Permethylation of Released Glycans C18 Clean-up and Mass Spectrometry Analysis
- 10:33Results: Recovery and Mass Spectral Analysis of Permethylated O-linked Glycans
- 12:30Conclusion
In order to comprehensively explore the diversity of O-linked glycans, a new procedure for in-gel reductive β-elimination, combined with permethylation and a rapid phase-partition method, is applied to the analysis of O-linked glycans directly released from glycoproteins resolved by SDS-PAGE and amenable to subsequent glycomic analysis by mass spectrometry.
