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Chapters
- 00:05Title
- 01:42Preparation for Receiving Frozen Samples
- 02:45High-pressure Freezing of Samples
- 06:22Preparation for Freeze Substitution
- 08:14Quick Freeze Substitution (QFS)
- 09:26Post FS Processing
- 10:45Results: Tandem HPF and QFS Produce Samples with High Quality Ultastructural Preservation
- 12:11Conclusion
Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.
