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במבחנת כינונה מחדש של מכלולי אור קציר של צמחים ואצות ירוקות
JoVE 신문
생물학
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JoVE 신문 생물학
In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae
DOI:

11:55 min

October 10, 2014

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Chapters

  • 00:05Title
  • 01:24Total Pigment Extraction from Spinach Leaves
  • 04:52Reconstitution
  • 07:37Nickel Column Purification
  • 08:18Sucrose Gradient Centrifugation
  • 09:21Results: Reconstituted LHCs Have the Same Properties as Native LHCs
  • 11:33Conclusion

Summary

자동 번역

This protocol details the reconstitution of light-harvesting complexes in vitro. These integral membrane proteins coordinate chlorophylls and carotenoids and are responsible for harvesting light in higher plants and green algae.

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