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通过鼻腔活检获得联合激光捕获显微切割的嗅觉神经元：一种可能的方法来研究治疗反应的精神障碍
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Olfactory Neurons Obtained through Nasal Biopsy Combined with Laser-Capture Microdissection: A Potential Approach to Study Treatment Response in Mental Disorders
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通过鼻腔活检获得联合激光捕获显微切割的嗅觉神经元：一种可能的方法来研究治疗反应的精神障碍

DOI:
10.3791/51853-v

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08:33 min

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December 04, 2014

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, , , , , , , , ,
1Department of Psychiatry,Johns Hopkins University, 2Department of Psychiatry and Behavioral Sciences,Howard University, 3Department of Otolaryngology-Head and Neck Surgery,Johns Hopkins University, 4Department of Psychiatry,Sheppard Pratt Hospital, 5Department of Psychiatry,Indiana University

Chapters

  • 00:05Title
  • 01:49Laser-Capture Microdissection
  • 03:48Isolation of Total RNA and cDNA Synthesis
  • 05:50Real Time PCR Quantification
  • 07:14Reults: GSK-3β Expression in OE Neurons
  • 07:53Conclusion

Summary

자동 번역

자동 번역

在这项研究中，一种新的平台来调查在躁郁症（BD）的治疗的反应的突触体分子签名被开发和验证。从BD患者嗅上皮是通过鼻腔活检获得。那么激光捕获显微切割是结合实时RT PCR，调查BD锂反应的分子签名。

Tags

Olfactory NeuronsNasal BiopsyLaser-capture MicrodissectionBipolar DisorderLithium CarbonateWnt SignalingNeuropsychiatric DisordersOlfactory EpitheliumTreatment Response

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