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Chapters
- 00:05Title
- 01:48Preparation of Equipment and Solutions
- 02:41Murine Valvular Dissection
- 05:16Cell Dissociation and Preparation for FACS
- 07:21Characterization of Isolated Valve Endothelial Cells
- 09:07Conclusion
The ability to isolate heart valve endothelial cells (VECs) is critical for understanding mechanisms of valve development, maintenance, and disease. Here we describe the isolation of VECs from embryonic and adult Tie2-GFP mice using FACS that will allow for studies determining the contribution of VECs in developmental and disease processes.
