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Chapters
- 00:05Title
- 01:35Mouse Brain Protein Extraction
- 03:25Telomeric Repeat Amplification Protocol (TRAP)
- 06:12Agarose Mini-gel Electrophoresis and Gel Staining
- 08:16Results: The Modified TRAP Assay is More Sensitive than the Traditional Assay in Detecting Telomerase Activity
- 09:45Conclusion
Telomerase is expressed in the neonatal brain and also in distinct regions of the adult brain. We present a non-toxic time saving TRAP assay for the analysis of telomerase activity in various regions of the mouse brain and detection of differences in telomerase activity between male and female mouse brains.
