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Chapters
- 00:05Title
- 02:12Cell Culture
- 03:36Setup of Hardware and Software for Cantilever Deflection Analysis
- 04:59Recording Cantilever Deflection Data
- 09:01Results: Analysis of Contractile Behavior in Real-time
- 10:30Conclusion
This protocol describes the use of microscale silicon cantilevers as pliable culture surfaces for measuring the contractility of muscle cells in vitro. Cellular contraction causes cantilever bending, which can be measured, recorded, and converted into readouts of force, providing a non-invasive and scalable system for measuring contractile function in vitro.
Tags
Keywords:
Microscale Silicon CantileversCellular Contractile FunctionIn Vitro AssaysCell Culture SystemsFunctional AssessmentContractile FunctionalitySkeletal MuscleCardiac MuscleSmooth MuscleContraction-induced Substrate BendingMultiplexed Cantilever ArraysDrug EfficacyDrug ToxicityDisease PhenotypeDisease ProgressionNeuromuscular InteractionsCell-cell InteractionsCantilever Array FabricationCell Culture MethodsFunctional AnalysisLaser-based Detection SystemContractile Function AnalysisDisease DevelopmentTherapeutic Response
