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विच्छेदन, इमेजिंग, और औषधीय उपचार: ड्रोसोफिला पोटा संबंधी वृषण और एकल पुरुष रोगाणु लाइन अल्सर के पूर्व vivo संस्कृति
JoVE 신문
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JoVE 신문 생물학
Ex vivo Culture of Drosophila Pupal Testis and Single Male Germ-line Cysts: Dissection, Imaging, and Pharmacological Treatment
DOI:

08:35 min

September 11, 2014

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Chapters

  • 00:05Title
  • 01:12Dissection of Pupal Testes
  • 03:40Dissection of Male Germ-line Cysts and Live Imaging
  • 04:58Pharmacological Assay with Intact Pupal Testes
  • 06:30Results: Dynamic Analysis of Drosophila Testes
  • 08:08Conclusion

Summary

자동 번역

This protocol describes the dissection and cultivation of intact testes and germ-line cysts from Drosophila melanogaster pupae. This method allows microscopic observation of spermatogenesis ex vivo. Furthermore, we describe a pharmacological assay of the effect of inhibitors on specific stages of germ-cell development in pupal testes.

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