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小鼠胎肝文化系统解剖靶基因功能的终端红细胞生成的早期和晚期

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis

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小鼠胎肝文化系统解剖靶基因功能的终端红细胞生成的早期和晚期

DOI:

10.3791/51894-v

•

06:40 min

•

September 09, 2014

•

Baobing Zhao, Yang Mei, Jing Yang, Peng Ji

¹Department of Pathology,Northwestern University

Chapters

00:05Title
01:21Purification of Fetal Liver Erythroblasts
02:32Purification of TER119 Negative Erythroblasts
03:50Transduced TER119 Negative Mouse Fetal Liver Erythroblast Culture
04:57Results: Representative Flow Cytometric Analyses of Differentiated and Enucleated Transduced Erythroblasts
06:08Conclusion

Summary

자동 번역

我们提出了一个在体外小鼠胎肝红细胞培养系统剖析终端红细胞生成的早期和晚期阶段。这个系统有利于在不同的发育阶段特异性基因的功能分析。

Tags

Mouse Fetal Liver Erythropoiesis Erythroid Burst Forming Units (BFU-E)Erythroid Colony Forming Units (CFU-E)Terminal Erythroblasts TER119 Erythroid Progenitor Cells Gene Function Analysis Early And Late Stages Of Terminal Erythropoiesis CDNA Overexpression ShRNA Knockdown Erythropoietin (Epo)

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