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Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
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Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

DOI:
10.3791/51896-v

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08:06 min

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September 03, 2014

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, , ,
1Department of Pharmacology and Toxicology,University of Toronto

Chapters

  • 00:05Title
  • 01:16Tissue Homogenization and Fractioning
  • 03:57Isolating the Synaptic Plasma Membrane (SPM) Fraction
  • 07:02Western Blot of Fractions
  • 07:32Conclusion

Summary

자동 번역

자동 번역

This article details the enrichment of proteins associated with the synaptic plasma membrane by ultracentrifugation on a discontinuous sucrose gradient. The subsequent preparation of post-synaptic density proteins is also described. Protein preparations are suitable for western blotting or 2D DIGE analysis.

Tags

Synaptic Plasma MembranePostsynaptic DensitySucrose GradientNeuronal Subcellular FractionationSynaptic Protein IsolationMembrane Protein IsolationPostsynaptic Density Protein IsolationWestern Blotting2D DIGE

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