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Raccolta, isolamento e analisi citofluorimetrica di campioni endocervicali umano
JoVE 신문
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Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
DOI:

12:34 min

July 06, 2014

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Chapters

  • 00:00Title
  • 02:22Isolation of Cervical Mononuclear Cells (CMCs)
  • 05:11CMC Surface Staining and Flow Cytometry
  • 08:06Collection and Preparation of Cervical-Vaginal Lavage (CVL)
  • 08:36Data Acquisition and Analysis
  • 11:56Conclusion

Summary

자동 번역

The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.

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