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Chapters
- 00:05Title
- 01:56Acute Dissociation of Lamprey Spinal Cord to Yield Isolated Reticulospinal Axons
- 05:26Labeling and Identification of Presynaptic Terminals with FM 1-43
- 06:15Electrophysiological Recording
- 07:51Immunohistochemistry of Isolated Reticulospinal Axons
- 09:32Results: Representative Recordings and Immunostaining of an Isolated Reticulospinal Axon
- 11:21Conclusion
Recording Ca2+ currents at the presynaptic release face membrane is key to a precise understanding of Ca2+ entry and neurotransmitter release. We present an acute dissociation of the lamprey spinal cord that yields functional isolated reticulospinal axons, permitting recording directly from the release face membrane of individual presynaptic terminals.
