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RNA-Seq Analyse der differentiellen Genexpression in elektroporiertem Küken Embryonale Spinal Cord

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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

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RNA-Seq Analyse der differentiellen Genexpression in elektroporiertem Küken Embryonale Spinal Cord

DOI:

10.3791/51951-v

•

11:13 min

•

November 01, 2014

•

Felipe M. Vieceli, C.Y. Irene Yan

¹Department of Cell and Developmental Biology,Universidade de São Paulo

Chapters

00:05Title
01:22Harvesting Neural Tubes for RNA Collection
04:32Pooling Tissue to Purify RNA for Illumina Sequencing
05:34Comparing RNA-Seq Datasets on the Galaxy Public Server
09:52Results: Transcriptome Analyis Quality
09:52Transcriptome Analysis Quality
10:42Conclusion

Summary

자동 번역

This video shows the basic steps for performing whole transcriptome analysis on dissected chick embryonic spinal cord samples after transfection with in ovo electroporation.

Tags

RNA-Seq Differential Gene Expression Chick Embryonic Spinal Cord In Ovo Electroporation Neural Development Transcriptome Analysis Gene Function Bioinformatics Galaxy Public Server High-quality RNA Samples Overexpression

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