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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis

DOI:
10.3791/51964-v

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12:55 min

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February 16, 2015

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, , ,
1W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 2School of Life Sciences,Chinese University of Hong Kong, 3Center for Cell Dynamics, Department of Biological Chemistry,Johns Hopkins University School of Medicine

Chapters

  • 00:05Title
  • 03:09Preparation of Cells for Live Cell Imaging
  • 04:38Stain Live Cells with Mitochondria and Nucleus-labeling Dyes
  • 06:09Live Cell Imaging to Detect Reversal of Apoptosis
  • 09:42Results: Tracking Reversal of Apoptosis by Live Cell Imaging
  • 11:46Conclusion

Summary

자동 번역

자동 번역

The term anastasis refers to the phenomenon in which dying cells reverse a cell suicide process at a late stage, repair themselves, and ultimately survive. Here we demonstrate protocols for detecting and tracking cells that undergo anastasis.

Tags

AnastasisCell SurvivalApoptosisCell DeathMitochondrial FragmentationCaspase ActivationChromatin CondensationDNA DamageCell Surface PhosphatidylserineApoptotic BodiesLive Cell MicroscopyCaspase Biosensor

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