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Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos
 

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Article DOI: 10.3791/51991-v 11:16 min September 24th, 2014
September 24th, 2014

챕터

요약

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

Tags

Keywords: Neuron Isolation Cell Culture Chick Embryo Sensory Neurons Primary Neurons Laminin Substrate In Vitro Model Immunocytochemistry Live Cell Imaging RNAi
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