JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 02:03Cell Spheroids Incubated with a Chemotherapeutic Drug
- 02:55Light Sheet Adjustment
- 04:09Cell Spheroid Application and Microscope Feed Synchronization
- 05:31Measurement in Dynamic Liquid Environment and Data Acquisition and Analysis
- 07:00Results: Imaging of Cell Spheroids Incubated with the Cytostatic Drug Doxorubicin
- 08:07Conclusion
A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.
