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Chapters
- 00:05Title
- 01:54Purification of Protein Arginine Methyl Transferases (PRMTs)
- 05:31Methylation Assay
- 07:16Confirmation of PRMT Expression and Equal Loading of Substrate
- 08:38Results: PRMT1 Methylates Ras-GTPase Activating Protein Binding Protein 1 (G3BP1)
- 09:40Conclusion
Protein arginine methylation, catalyzed by a class of enzymes viz., protein arginine methyl transferases (PRMTs), is the process of enzymatic addition of methyl group(s) to arginines within proteins. The in vitro methylation assay is the most dependable tool for assessing the methylation status of known or novel PRMT substrates.
