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Dyrking av pattedyrceller med en enkelt-bruk Pneumatisk Bioreaktor System
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생체공학
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Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System

Dyrking av pattedyrceller med en enkelt-bruk Pneumatisk Bioreaktor System

DOI:
10.3791/52008-v

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11:42 min

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October 10, 2014

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, , , ,
1Center for Biotechnology Education,Johns Hopkins University, 2PBS Biotech, Inc.

Chapters

  • 00:05Title
  • 01:35Login and Calibration
  • 03:41Autoclave, Sensor, and Reagent Vessel Installation
  • 06:43Medium- and Micro-carriers Addition, Equilibration, and One-point DO Calibration
  • 08:28Run Initiation, Cell Inoculation, and Sampling
  • 10:11Results: Characterization and Analysis of Bioreactor Cell Culture and Viral Growth
  • 11:12Conclusion

Summary

자동 번역

자동 번역

Using a pneumatic bioreactor, we demonstrate the assembly, operation, and performance of this single-use bioreactor system for the growth of mammalian cells.

Tags

Keywords: Mammalian Cell CultivationSingle-use BioreactorPneumatic BioreactorCell Culture Scale-upBioreactor MonitoringCell Growth OptimizationTherapeutic Protein ProductionMonoclonal Antibody ProductionStem Cell ExpansionBiosimilar ManufacturingVaccine Production

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