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High Efficiency Differentiering av Human pluripotenta stamceller till Cardiomyocytes och karakterisering av flödescytometri
 
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High Efficiency Differentiering av Human pluripotenta stamceller till Cardiomyocytes och karakterisering av flödescytometri

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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Artikeln beskriver detaljerad metod för att effektivt differentiera humana pluripotenta stamceller till kardiomyocyter genom att selektivt modulera Wnt pathway, följt av flödescytometrianalys av referensmarkörer för att bedöma homogenitet och identitet av befolkningen.

Tags

Cellular Biology mänskliga inducerade pluripotenta stamceller flödescytometri riktad differentiering cardiomyocyte IRX4 TNNI3 TNNT2 MCL2v MLC2a
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