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白细胞亚群在暂时或永久的脑缺血模型体视学和流式细胞仪鉴定

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Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia

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白细胞亚群在暂时或永久的脑缺血模型体视学和流式细胞仪鉴定

DOI:

10.3791/52031-v

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12:42 min

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December 28, 2014

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Iván Ballesteros*¹, María Isabel Cuartero*¹, Ana Moraga, Juan de la Parra, Ignacio Lizasoain, María Ángeles Moro

¹Unidad de Investigacón Neurovascular, Departmento de Farmacología, Falcultad de Medicina,Universidad Complutense de Madrid y Instituto de Investigación Hospital 12 de Octubre, Madrid

Chapters

00:05Title
02:20Common Carotid Artery and Distal Middle Cerebral Artery Occlusion by Ligation
03:56Perfusion and Sectioning of Brain Tissue
05:36Stereological Quantification of Infiltrated Neutrophils After Cerebral Ischemia by Optical Fractionation
07:23Brain Dissociation and Single Cell Suspension Preparation
09:41Results: Representative Flow Cytometric Analysis of Brain Leukocytes Obtained from Naïve, Sham and Ischemic Mice
11:44Conclusion

Summary

자동 번역

脑的骨髓细胞的表征下列行程可以通过体视用光学分馏方法来进行，或者通过大脑的流式细胞仪分析白细胞悬浮液。两者都是有用的技术来执行，在缺血性脑中发现的主要的骨髓细胞亚群的准确的表型区分。

Tags

Keywords: Stereological Analysis Flow Cytometry Leukocyte Subpopulations Cerebral Ischemia Microglia Macrophages Neutrophils Brain Injury Myeloid Cells Cavalieri Method Optical Fractionator

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