Journal
/
생물학
/
Osteoclast Derivation from Mouse Bone Marrow
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Osteoclast Derivation from Mouse Bone Marrow

Osteoclast Derivation from Mouse Bone Marrow

DOI:
10.3791/52056-v

•

06:17 min

•

November 06, 2014

•

, , , , , , , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Chapters

  • 00:05Title
  • 01:28Media Preparation and Bone Marrow Isolation
  • 03:05Gradient Separation of Bone Marrow Cells and Cell Culturing
  • 04:57Results: Culturing Osteoclasts from Mouse Bone Marrow
  • 05:56Conclusion

Summary

자동 번역

자동 번역

Osteoclasts are the principal bone-resorbing cell in the body. An ability to isolate osteoclasts in large numbers has resulted in significant advances in the understanding of osteoclast biology. In this protocol, we describe a method for isolation, cultivating and quantifying osteoclast activity in vitro.

Tags

OsteoclastBone MarrowMonocyte/macrophage LineageBone ResorptionBone RemodelingOsteoblastsSkeletal RemodelingBone MatrixBone FormationBone DiseaseOsteoporosisIn Vitro Osteoclast IsolationBone Remodeling CycleTherapeutic Strategies

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Culture of myeloid dendritic cells from bone marrow precursors

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

Homing of Hematopoietic Cells to the Bone Marrow

Isolation and Derivation of Mouse Embryonic Germinal Cells

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

Generation of Mice Derived from Induced Pluripotent Stem Cells

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Proplatelet Formation Dynamics of Mouse Fresh Bone Marrow Explants

Read Article