This content is Free Access.
Chapters
- 00:05Title
- 02:12Isolation and Selection of T. castaneum Larvae
- 03:35Preparation of Injection Needles
- 04:39Frontloading dsRNA Solution into the Needle
- 06:06dsRNA Injection
- 08:05Results: Microinjections of T. castaneum
- 09:52Conclusion
RNA interference (RNAi)-based gene knockdown techniques are at the core of Tribolium research. Here, we provide an overview of our larval RNAi technique in Tribolium castaneum. Larval RNAi is a simple, but powerful technique that provides quick access to loss-of-function phenotypes, allowing researchers to study gene functions in diverse contexts.
