Journal
/
/
Slice It Hot: Agudo Adulto Cérebro Cortando na temperatura fisiológica
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature
DOI:

08:46 min

October 30, 2014

, ,

Chapters

  • 00:05Title
  • 01:17Setting Up for the Slicing Procedure
  • 02:53Dissecting the Brain
  • 04:52Brain Slicing
  • 07:09Results: Patch-clamp Recordings from Cerebellar Neurons
  • 08:25Conclusion

Summary

자동 번역

In this paper we show a method for preparing acute brain slices in physiological temperature, using a conventional physiological solution without special modifications for the cutting (such as adding sucrose) and without intracardial perfusion of the animal before slice preparation.

Related Videos

Read Article