Journal
/
생물학
/
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

DOI:
10.3791/52095-v

•

14:15 min

•

November 18, 2014

•

,
1Molecular, Cellular, and Developmental Biology,University of Colorado, Boulder, 2Linda Crnic Institute for Down Syndrome,University of Colorado, Denver

Chapters

  • 00:05Title
  • 01:45Adenylation of 3′ Linker
  • 05:14Linker Ligation to 3′ End of miRNA
  • 10:29Linker Ligation to 5′ End of miRNA-3′ Linker Hybrid
  • 11:46Results: Ligation of Small RNA Molecules by the Method is Highly Efficient
  • 13:50Conclusion

Summary

자동 번역

자동 번역

MicroRNAs (miRNAs) are a widely conserved class of regulatory molecules. Here we describe a miRNA cloning method that relies upon two potent ligation steps followed by high-throughput sequencing. Our method permits accurate genome-wide quantitation of miRNAs.

Tags

MiRNAMiR-SeqHigh-throughput SequencingSmall RNA LigationLibrary ConstructionQuantitationBias ReductionTranscriptome-wide Profiling

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/52095?article_type=v

Gramicidin-based Fluorescence Assay; for Determining Small Molecules Potential for Modifying Lipid Bilayer Properties
check_url/kr/52095?article_type=v

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
check_url/kr/52095?article_type=v

Substrate Generation for Endonucleases of CRISPR/Cas Systems
check_url/kr/52095?article_type=v

RNA Secondary Structure Prediction Using High-throughput SHAPE
check_url/kr/52095?article_type=v

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
check_url/kr/52095?article_type=v

A Manual Small Molecule Screen Approaching High-throughput Using Zebrafish Embryos
check_url/kr/52095?article_type=v

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
check_url/kr/52095?article_type=v

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
check_url/kr/52095?article_type=v

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
check_url/kr/52095?article_type=v

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Read Article