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Chapters
- 00:05Title
- 01:34Designing a Peptide Array and Blocking Non-specific Binding
- 03:04Incubating the Array with the Protein and Antibody
- 05:09Reading the Array
- 05:57Results: Screening a Peptide Array for Binding of STIL Intrinsically Disordered Region to CHFR Protein
- 07:18Conclusion
Peptide array screening is a high throughput assay for identifying protein-protein interaction sites. This allows mapping multiple interactions of a target protein and can serve as a method for identifying sites for inhibitors that target a protein. Here we describe a protocol for screening and analyzing peptide arrays.
