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Langsigtet Time Lapse Imaging af Mouse Cochlear Eksplantater
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Long-term Time Lapse Imaging of Mouse Cochlear Explants

Langsigtet Time Lapse Imaging af Mouse Cochlear Eksplantater

DOI:
10.3791/52101-v

•

10:43 min

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November 02, 2014

•

,
1Biological Sciences Platform,Sunnybrook Research Institute, 2Department of Otolaryngology – Head and Neck Surgery,University of Toronto, 3Department of Laboratory Medicine and Pathobiology,University of Toronto

Chapters

  • 00:05Title
  • 01:31Culturing Embryonic Cochleae and Explants
  • 05:56Live Imaging
  • 08:57Results: Cochlear Explant Time Lapse
  • 10:06Conclusion

Summary

자동 번역

자동 번역

Live-billeddannelse af embryonale pattedyr cochlea er udfordrende, fordi de udviklingsmæssige processer ved hånden operere på en tidsmæssig gradient i løbet af ti dage. Her præsenteres en fremgangsmåde til dyrkning og derefter afbilde embryonale cochlear eksplantat væv taget fra en fluorescerende reporter musen over fem dage.

Tags

Cochlear ExplantTime-lapse ImagingLive ImagingEmbryonic MouseMammalian CochleaGene ExpressionFluorescent MicroscopyBright Field MicroscopyIncubator MicroscopeSox2Prosensory Cells

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