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Identifikation af Nøglefaktorer Regulering Self-fornyelse og differentiering i EML Hæmatopoietisk precursorceller ved RNA-sekventering Analysis
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identifikation af Nøglefaktorer Regulering Self-fornyelse og differentiering i EML Hæmatopoietisk precursorceller ved RNA-sekventering Analysis

DOI:
10.3791/52104-v

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12:44 min

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November 11, 2014

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, , ,
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center,The University of Texas Graduate School of Biomedical Sciences at Houston

Chapters

  • 00:05Title
  • 02:09Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
  • 03:25EML Cell Culture and Depletion of Lineage Positive Cells
  • 04:52Separation of Lin–CD34+ and Lin–CD34– Cells
  • 07:15RNA Preparation and Library Construction for High-throughput Sequencing
  • 09:54Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
  • 11:38Conclusion

Summary

자동 번역

자동 번역

RNA-sekventering og bioinformatik analyser blev anvendt til at identificere betydeligt, differentielt udtrykte transkriptionsfaktorer i Lin-CD34 + Lin-CD34 subpopulationer af mus EMLcells. Disse transkriptionsfaktorer kan spille en vigtig rolle i fastsættelsen af ​​kontakten mellem selvfornyende Lin-CD34 + og delvist differentierede Lin-CD34-celler.

Tags

Keywords: RNA-sequencingEML CellsHematopoietic Stem CellsSelf-renewalDifferentiationTranscription FactorsGene ExpressionLin-CD34+ CellsLin-CD34- CellsRNA IsolationLibrary ConstructionData Analysis

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