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High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability
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High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability

High Resolution Electron Microscopy of the Helicobacter pylori Cag Type IV Secretion System Pili Produced in Varying Conditions of Iron Availability

DOI:
10.3791/52122-v

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09:05 min

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November 21, 2014

•

, ,
1Department of Medicine – Division of Infectious Diseases,Vanderbilt University School of Medicine, 2Tennessee Valley Healthcare Systems,U. S. Dept. of Veterans Affairs

Chapters

  • 00:05Title
  • 01:36H. pylori, Iron Availability and AGS Co-culture
  • 03:24SEM Sample Preparation
  • 05:01Imaging Parameters for High Resolution SEM Analyses
  • 05:46Quantifying H. pylori Cag-T4SS Pili
  • 07:07Results: Effect of Iron on H. pylori Cag-T4SS Pilus Biogenesis
  • 08:31Conclusion

Summary

자동 번역

자동 번역

Here we describe a method to visualize the oncogenic bacterial organelle known as the Cag Type IV Secretion System (Cag-T4SS). We find that the Cag-T4SS is differentially produced on the surface of H. pylori in response to varying conditions of iron availability.

Tags

Keywords: Helicobacter PyloriCag Type IV Secretion SystemPiliIron AvailabilityScanning Electron MicroscopyVirulence FactorGastric Cancer

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