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Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease
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Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease

Isolation and Excision of Murine Aorta; A Versatile Technique in the Study of Cardiovascular Disease

DOI:
10.3791/52172-v

•

07:25 min

•

November 24, 2014

•

, , ,
1Department of Emergency Medicine,University of Cincinnati College of Medicine

Chapters

  • 00:05Title
  • 01:12Isolating the Heart and Aorta
  • 03:08Perfusion of the Heart and Aorta
  • 04:05Isolation of the Aorta and Other Tissues
  • 06:03Results: Analysis of the Isolated Aorta
  • 07:03Conclusion

Summary

자동 번역

자동 번역

Pathology of the aorta can lead to severe morbidity and mortality, therefore research of disease progression and potential therapies is warranted. Here, we present a protocol to isolate and excise the murine aorta to aid researchers in their investigation of cardiovascular disease.

Tags

Keywords: AortaCardiovascular DiseaseAtherosclerosisAneurysmIsolationExcisionMorphometric ChangesHistologyMolecular StudiesProtein ExpressionGene ExpressionPrimary CellsIn Situ AssaysIn Vitro Assays

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