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Chapters
- 00:05Title
- 01:43Preparations
- 03:15Dissection of Adult Murine Cervical Spinal Cord
- 06:36Nile Red Labeling and Imaging with Laser–induced Injury
- 09:01Results: Dynamic Injury Response Recordings
- 10:31Conclusion
We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.
