Journal
/
/
Üretim ve seçici transduce İlköğretim Oligodendrosit Öncü Hücreler için Lentivirüste kullanımı için<em> İn Vitro</em> Myelinasyon Tahliller
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Production and Use of Lentivirus to Selectively Transduce Primary Oligodendrocyte Precursor Cells for In Vitro Myelination Assays
DOI:

10:00 min

January 12, 2015

, , , ,

Chapters

  • 00:05Title
  • 01:242K7 Lentivirus Production
  • 05:07Transducing OPCs and Seeding for Co-culture
  • 08:07Results: Transformed OPCs Differentiating on DRGs
  • 09:32Conclusion

Summary

자동 번역

Here we present protocols that offer a flexible and strategic foundation for virally manipulating oligodendrocyte precursor cells to overexpress proteins of interest in order to specifically interrogate their role in oligodendrocytes via the in vitro model of central nervous system myelination.

Related Videos

Read Article