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대 식세포 식균 작용과 액틴의 중합의 평가를위한 높은 처리량 형광 측정 기법
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
DOI:

09:22 min

November 27, 2014

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Chapters

  • 00:05Title
  • 01:38Plating and Treating Cells
  • 02:16Opsonization of Flourescent Particles
  • 03:36Phagocytosis
  • 05:08Actin Staining
  • 06:10Quantification
  • 07:33Results: Confocal Microscopy of Phagocytosis and Actin Polymerization
  • 08:47Conclusion

Summary

자동 번역

Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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