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Chapters
- 00:05Title
- 01:54Preparation of Peptide Arrays
- 04:48Peptide Array Methylation
- 06:18Results: Characterization of Methylated Peptide Arrays
- 07:38Conclusion
Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.
