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Chapters
- 00:05Title
- 02:36Initial Agarose Pad Preparation for Fluorescent Microscopy
- 03:29Biomolecule Incorporation via Electroporation
- 07:14Fluorescent Microscopy: Data Acquisition
- 09:07Fluorescent Microscopy: General Data Analysis
- 10:42Fluorescent Microscopy: Cell-based Photobleaching Analysis
- 14:11Conclusion
Studies of biomolecules in vivo are crucial for understanding molecular function in a biological context. Here we describe a novel method allowing the internalization of fluorescent biomolecules, such as DNA or proteins, into living microorganisms. Analysis of in vivo data recorded by fluorescence microscopy is also presented and discussed.
