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Detektion og analyse af DNA-skader i Mouse Skeletmuskel<em> In Situ</em> Brug af TUNEL Method
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Detection and Analysis of DNA Damage in Mouse Skeletal Muscle In Situ Using the TUNEL Method

Detektion og analyse af DNA-skader i Mouse Skeletmuskel<em> In Situ</em> Brug af TUNEL Method

DOI:
10.3791/52211-v

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09:54 min

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December 16, 2014

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,
1Division of Neuropathology, Department of Pathology, Pathobiology Graduate Program,Johns Hopkins School of Medicine

Chapters

  • 00:05Title
  • 01:01Fixing, Embedding and Cryosectioning Neonatal Hindlimb Tissue
  • 02:34TUNEL Assay and Fluorescent Labeling
  • 06:02Imaging and Image Analysis
  • 08:01Results: Measuring Apoptosis in a Mouse Model of Spinal Muscular Atrophy
  • 09:34Conclusion

Summary

자동 번역

자동 번역

This video describes dissection, tissue processing, sectioning, and fluorescence-based TUNEL labeling of mouse skeletal muscle. It also describes a method for semi-automated analysis of TUNEL labeling.

Tags

TUNELDNA DamageMouseSkeletal MuscleFluorescenceApoptosisQuantitation

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