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Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells
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Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells

Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells

DOI:
10.3791/52214-v

•

07:07 min

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March 08, 2015

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, , ,
1Department of Cell and Molecular Biology,The Scripps Research Institute, 2Lowy Medical Research Institute

Chapters

  • 00:05Title
  • 01:47Directed Differentiation of Stem Cell-derived Retinal Pigment Epithelium (RPE)
  • 02:51Isolating Pigmentation Islets
  • 03:59Passaging Stem Cell-derived RPE
  • 05:42Results: Representative iPS-RPE Culture and Expansion
  • 06:19Conclusion

Summary

자동 번역

자동 번역

Stem cell-derived retinal pigment epithelium (RPE) cells may be used for multiple applications including cell-based therapies for retinal degeneration, disease modeling, and drug studies. Here we present a simple protocol for reproducibly deriving RPE from stem cells.

Tags

Keywords: Retinal Pigment EpitheliumRPEAge-related Macular DegenerationAMDStem Cell DifferentiationIPS-RPENicotinamideActivin AIDE-1Cell-based Therapy

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