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Chapters
- 00:05Title
- 01:40Extraction of M1 (Pro-inflammatory) and M2 (Anti-inflammatory) Macrophage Proteins for 2D Electrophoresis
- 03:55Isoelectrofocusing and Second Dimensional Electrophoresis
- 05:29Image Acquisition and Bioinformatic Analysis
- 06:25Results: Bioinformatic Analysis of M1 and M2 2D Protein Patterns
- 07:15Conclusion
Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.
