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神经肌肉接头处：测量突触大小，碎片和改变突触蛋白密度利用共聚焦荧光显微镜

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The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

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神经肌肉接头处：测量突触大小，碎片和改变突触蛋白密度利用共聚焦荧光显微镜

DOI:

10.3791/52220-v

•

12:18 min

•

December 26, 2014

•

Nigel Tse, Marco Morsch, Nazanin Ghazanfari, Louise Cole, Archunan Visvanathan, Catherine Leamey, William D. Phillips

¹Physiology and Bosch Institute,University of Sydney, ²Motor Neuron Disease Research Group, Australian School of Advanced Medicine,Macquarie University, ³Advanced Microscopy Facility, Bosch Institute,University of Sydney

Chapters

00:05Title
01:48Unbiased Sampling and En Face Imaging of Motor Endplates
03:41Measuring the Area of Synaptic Specializations in en face Images
07:36Relative Staining Intensities Compared Using Transverse Optical Sections
09:47Results: Measurement of Synaptic Area at the Neuromuscular Junction
11:29Conclusion

Summary

자동 번역

The neuromuscular junction (NMJ) is altered in a variety of conditions that can sometimes culminate in synaptic failure. This report describes fluorescence microscope-based methods to quantify such structural changes.

Tags

Keywords: Neuromuscular Junction Synapse Confocal Microscopy Acetylcholine Receptor Synaptic Vesicle Synaptic Protein FRET Neurotransmission Muscle Contraction Structural Changes Mouse Model

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