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Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro
 

Method for the Assessment of Effects of a Range of Wavelengths and Intensities of Red/near-infrared Light Therapy on Oxidative Stress In Vitro

Article DOI: 10.3791/52221-v • 08:16 min • March 21st, 2015
March 21st, 2015 •
1, 2, 1, 3, 3, 1
1Experimental and Regenerative Neurosciences, School of Animal Biology, The University of Western Australia, 2School of Animal Biology and The Oceans Institute, The University of Western Australia, 3Experimental and Regenerative Neurosciences, School of Anatomy, Physiology and Human Biology, The University of Western Australia

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Non-coherent Xenon light was passed through narrow-band interference and neutral density filters to deliver light of varying wavelength and intensity to cultured cells. This protocol was used to assess the effects of red/near-infrared light therapy on production of reactive species in vitro: no effects were observed using the tested parameters.

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Keywords: Red/near-infrared Light Therapy Wavelength Intensity Oxidative Stress Reactive Oxygen Species In Vitro Cell Culture Glutamate DCFH-DA H2O2 Light Delivery Apparatus Optimization
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