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Chapters
- 00:05Title
- 01:35Cell Sorting and Plating
- 04:16Serial Sphere Passaging
- 05:59Results: Representative RFP+ and RFP- tumor Sphere Imaging
- 07:56Conclusion
In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.
